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Fig. 5 | 3D Printing in Medicine

Fig. 5

From: Trabecular-bone mimicking osteoconductive collagen scaffolds: an optimized 3D printing approach using freeform reversible embedding of suspended hydrogels

Fig. 5

Immunocytochemistry of hOBs and SaOS-2 cells grown on FRESH-printed collagen scaffolds. Maximum intensity z projection images captured by confocal microscopy of hOBs and SaOS-2 cells grown on collagen scaffolds for 1 week (A) or 4 weeks (B), and stained for cell nuclei (DAPI), cytosol (carboxyfluorescein diacetate [CFDA]), and the osteoblast marker osteocalcin (OCN). hOBs and SaOS-distribution along the z axis after 1 week (C) or 4 weeks (D). (C-D) Images obtained from maximal intensity x projections using the CFDA channel. Scale bars: 200 μm (A-B), 100 μm (C-D). Both hOBs and SaOS-2 cells colonized the scaffolds within a 4-week timeframe. hOB, human osteoblast

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3D Printing in Medicine

ISSN: 2365-6271

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